Measured protein landscapes: 2026 study
Research context
study · conditional · As of 2026-10-10
peer reviewed · 2026-05-13
- Study design
- Multiplexed intact-domain LC–IMS-MS hydrogen exchange with 13-domain HDX-NMR validation.
- Reported result
- Finds distinct opening-energy distributions among similar small folds and tests stabilizing mutations.
- Limitations
- EX2, pH and back-exchange assumptions plus domain-size/measurement selection limit transfer to intact cellular proteins.
Ferrari and colleagues profile 5,778 small domains with multiplexed HDX-MS and compare 13 domains with site-resolved HDX NMR.
Key knowledge
Ferrari and colleagues profile 5,778 small domains with multiplexed HDX-MS and compare 13 domains with site-resolved HDX NMR. Domains with similar folds/global stability can have different local opening behavior; the measured landscape distributions remain conditional on exchange and selection models.
Conditions and scope
- The analyzed domains are 28–64 residues, with expression/signal/QC selection; intact-domain rates are not residue-labeled trajectories of every full-length protein.
Sources · 1
References
Sources retain their recorded scope. A source link alone does not establish verification of every statement.
Evidence and review
Large-scale discovery, analysis and design of protein energy landscapes: mHDX-MS equations (1)–(5), EX2/pH/back-exchange assumptions and NMR validation (full relevant original method/results sections)
2026-10-10 · Bounded author source inspection of measured protein landscapes: 2026 study, including the stated conditions; independent branch review is recorded separately.
Entry details
Permanent link to this entry ↗- Record identity
- res-bio-energy-landscapes-2026
- Revision
- research.1
- Structured reference record
- Postgraduate & research reference