Genome-wide break detection and assay limits
Research context
research method · established · As of 2026-10-10
GUIDE-seq detects tagged double-strand-break locations by oligonucleotide integration and sequencing.
Key knowledge
GUIDE-seq detects tagged double-strand-break locations by oligonucleotide integration and sequencing. Its original human-cell-line experiments show variable off-target cleavage across guides; failure to detect a site is not proof of zero damage in every tissue or editing chemistry.
Conditions and scope
- Detection depends on tagging, delivery, cell/repair context and sequencing; DSB capture is not a complete assay for base substitutions, all structural changes or long-term safety.
Sources · 1
References
Sources retain their recorded scope. A source link alone does not establish verification of every statement.
Evidence and review
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases: Original abstract: double-stranded-oligonucleotide capture into DNA breaks and two-cell-line comparisons (original abstract and figure descriptions)
2026-10-10 · Bounded author source inspection of genome-wide break detection and assay limits, including the stated conditions; independent branch review is recorded separately.
Entry details
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- res-bio-guideseq-scope
- Revision
- research.1
- Structured reference record
- Postgraduate & research reference