Biological replication in single-cell inference
Research context
research method · established · As of 2026-10-10
Cells sampled from one animal or culture share experimental history.
Key knowledge
Cells sampled from one animal or culture share experimental history. Squair and colleagues show that treating such cells as independent biological replicates can inflate differential-expression discoveries; sample-level aggregation or appropriate hierarchical models preserve the treatment unit.
Conditions and scope
- More cells improve within-sample description but do not replace independent samples; conclusions are bounded by the tested designs and methods.
Sources · 1
References
Sources retain their recorded scope. A source link alone does not establish verification of every statement.
Evidence and review
Confronting false discoveries in single-cell differential expression: Benchmark results, biological pseudoreplication and pseudobulk controls (indexed primary results and methodological passages)
2026-10-10 · Bounded author source inspection of biological replication in single-cell inference, including the stated conditions; independent branch review is recorded separately.
Entry details
Permanent link to this entry ↗- Record identity
- res-bio-singlecell-units
- Revision
- research.1
- Structured reference record
- Postgraduate & research reference