SDS-PAGE and immunoblot evidence
SDS-PAGE uses denaturing detergent treatment to separate suitable proteins primarily by apparent size.
Key knowledge
SDS-PAGE uses denaturing detergent treatment to separate suitable proteins primarily by apparent size. Immunoblotting transfers separated material to a support and detects antibody binding. A band is assay evidence about a target under specified preparation and antibody conditions, rather than an automatic proof of molecular identity or activity.
Conditions and scope
- Reduction, extraction and anomalous migration affect apparent size.
- Antibody specificity, loading/transfer and detection range need controls before comparing abundance.
Sources · 1
References
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Evidence and review
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Entry details
Permanent link to this entry ↗- Record identity
- uni-bio-protein-assays
- Revision
- core.1
- Structured reference record
- University core expansion