Internal-standard response ratios
A distinct internal standard added to standards and samples permits calibration of SA/SIS=K cA/cIS, where K is the relative response.
Key knowledge
A distinct internal standard added to standards and samples permits calibration of SA/SIS=K cA/cIS, where K is the relative response. Ratios can reduce shared proportional preparation or measurement variation.
Values and equations
SA/SIS = K cA/cIS
- SA,SIS
- analyte and internal-standard signals compatible signal units
- K
- ratio of analytical sensitivities 1
- cA,cIS
- analyte and internal-standard concentrations compatible concentration units
Conditions and scope
- Both species need compatible variation and a stable relative response.
- Interferences and differential recovery are not automatically removed by ratioing.
Sources · 1
References
Sources retain their recorded scope. A source link alone does not establish verification of every statement.
Evidence and review
Read internal-standard equation 5.12 and proportional-variation example.
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Entry details
Permanent link to this entry ↗- Record identity
- uni-chem-internal-standard
- Revision
- core.1
- Structured reference record
- University core expansion