Cas9 cleavage: original biochemical evidence
Research context
study · established · As of 2026-10-10
peer reviewed · 2012-08-17
- Study design
- Purified-protein/RNA cleavage and nuclease-domain experiments.
- Reported result
- Demonstrates programmable dual-RNA-guided cleavage and a chimeric single-guide design.
- Limitations
- In-vitro cleavage does not quantify cellular efficiency, off-target safety or clinical benefit.
Jinek and colleagues demonstrated RNA-programmable Cas9 double-stranded DNA cleavage in vitro and a fused guide-RNA architecture.
Key knowledge
Jinek and colleagues demonstrated RNA-programmable Cas9 double-stranded DNA cleavage in vitro and a fused guide-RNA architecture. For the studied system, guide complementarity, PAM and separate nuclease domains specify recognition/cleavage; the biochemical experiment does not establish universal editing outcomes in cells.
Conditions and scope
- Original scope is purified S. pyogenes Cas9 biochemistry; cellular repair and delivery require separate evidence.
Sources · 1
References
Sources retain their recorded scope. A source link alone does not establish verification of every statement.
Evidence and review
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity: Biochemical cleavage, crRNA/tracrRNA fusion, HNH/RuvC and PAM dependence (full relevant original biochemical study sections)
2026-10-10 · Bounded author source inspection of cas9 cleavage: original biochemical evidence, including the stated conditions; independent branch review is recorded separately.
Entry details
Permanent link to this entry ↗- Record identity
- res-bio-cas9-mechanism
- Revision
- research.1
- Structured reference record
- Postgraduate & research reference