Prime editing and programmed repair intermediates
Research context
research method · established · As of 2026-10-10
Prime editing combines a Cas9 nickase–reverse-transcriptase fusion with a pegRNA carrying the desired edit.
Key knowledge
Prime editing combines a Cas9 nickase–reverse-transcriptase fusion with a pegRNA carrying the desired edit. Reverse-transcribed sequence and subsequent repair determine products; an additional nick can improve editing but also alter indel outcomes.
Conditions and scope
- PAM/pegRNA design, edit distance, delivery and cell state matter; proof in tested cells is not a universal efficacy claim.
Sources · 1
References
Sources retain their recorded scope. A source link alone does not establish verification of every statement.
Evidence and review
Search-and-replace genome editing without double-strand breaks or donor DNA: PegRNA, Cas9 nickase/reverse transcriptase, PE3 and cell-context outcomes (full relevant original mechanism/results sections)
2026-10-10 · Bounded author source inspection of prime editing and programmed repair intermediates, including the stated conditions; independent branch review is recorded separately.
Entry details
Permanent link to this entry ↗- Record identity
- res-bio-prime-editing
- Revision
- research.1
- Structured reference record
- Postgraduate & research reference