Sequence determination and assembly
Sanger sequencing detects terminated DNA products produced when extension incorporates a nucleotide lacking the required 3′ hydroxyl.
Key knowledge
Sanger sequencing detects terminated DNA products produced when extension incorporates a nucleotide lacking the required 3′ hydroxyl. Shotgun approaches sequence fragments and reconstruct overlaps computationally. A sequence result requires quality assessment; assembly and annotation are distinct inferences beyond raw detection.
Conditions and scope
- Modern sequencing platforms use multiple chemistries; they do not all rely on chain termination.
- Repeats, contamination, sampling and read errors can create gaps or incorrect assemblies.
Sources · 1
References
Sources retain their recorded scope. A source link alone does not establish verification of every statement.
Evidence and review
Read chain-termination and shotgun/assembly paragraphs; excluded obsolete cost/time/history claims and universal termination-chemistry statement.
2026-10-10 · Independent AI editorial review: All 115 actual compact claims, hypotheses, equation/variable/unit tables and 251 origin semantic links independently read. All exact author source-binding rows and disclosed extents assessed; targeted original bodies or specifically identified indexed primary passages rechecked below. All retained biology compact records read for combined breadth. Independent AI editorial content and practical common-core inventory review; no human specialist, degree/laboratory proficiency, whole-textbook certification or experimental replication. Receipt: biology-independent-content-inventory-review.1.
Entry details
Permanent link to this entry ↗- Record identity
- uni-bio-sequencing
- Revision
- core.1
- Structured reference record
- University core expansion