Electrophoretic and electroosmotic migration
In a buffered capillary, an analyte's velocity combines electrophoretic migration with electroosmotic solvent motion: v=(μep+μeo)E.
Key knowledge
In a buffered capillary, an analyte's velocity combines electrophoretic migration with electroosmotic solvent motion: v=(μep+μeo)E. Charge, molecular friction, buffer and wall chemistry affect mobility. Neutral species share solvent motion unless another separation mechanism acts.
Values and equations
v = (μep+μeo)E
- v
- signed analyte velocity m s⁻¹
- μep,μeo
- electrophoretic and electroosmotic mobilities m² V⁻¹ s⁻¹
- E
- signed axial electric field V m⁻¹
Conditions and scope
- Signed mobilities use a stated axial convention; wall treatment can reverse or suppress electroosmosis.
- The linear relation assumes the applicable mobility regime; heating and interactions can change response.
Sources · 1
References
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Evidence and review
Read equations 12.1/12.3/12.6, wall double-layer origin and neutral migration; excluded electroosmotic Gaussian-unit expression and dimensionally inconsistent plate-count rearrangement.
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Entry details
Permanent link to this entry ↗- Record identity
- uni-chem-capillary-electrophoresis
- Revision
- core.1
- Structured reference record
- University core expansion