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Biology · Research Design, Data & Scientific Practice

Reproducibility, open methods, literature evaluation and collaboration

A second reader obtains 4.5 from the same four eligible values and the same inclusion rule. A different reader obtains 5.5 from four new invented observations. These results describe different checks, even though both use the same averaging formula.

Life, Evidence & Biological Scale introduces evidence and biological organisation. Organisms, Environments & Ecological Niches and Population Ecology & Life-History Patterns develop the importance of boundaries and denominators. Their English chapters are available in this working manuscript; their publication remains pending. The examples here require arithmetic, averages and ordinary graph reading. The sample-variance and confidence-interval example explains its notation as it is introduced; it can be revisited after the simpler comparisons.

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By the end, you can

Distinguish recomputing the same analysis from collecting new evidence, evaluate literature by its methods and scope, and preserve planned versus exploratory analysis.

01

State what was reproduced

This lesson uses computational reproducibility for obtaining consistent results from the same input data, methods and analysis conditions. It uses replicability for consistency across studies answering the same question with their own data. Other communities use these words differently, so the convention and the actual work should be stated.

Recomputing 4.5 from the retained four eligible values checks the calculation and inclusion rule. Recomputing 3.6 after replacing the missing record with zero instead follows a different analysis. A difference need not be a failure of the original arithmetic; the two readers may have used different inputs or rules.

The new observations five, seven, nine and one have mean 5.5. They are new evidence in this imaginary example. Whether real studies support consistent conclusions depends on design, population, uncertainty and context, rather than exact equality of two sample means. Successful same-data computation also cannot repair a biased original sample.

A changed analysis differs from new evidence

A changed analysis differs from new evidenceFour named records separate the original summary and its exact recomputation from a changed missing-value rule and a distinct new-data record.Assigned response unitsOriginal namedsummaryOriginal named summary: 4.5Same inputs and rulerecomputedSame inputs and rule recomputed: 4.5Missing entryassumed to be zeroMissing entry assumed to be zero: 3.6A new inventedobservation recordA new invented observation record: 5.501.534.56Different missing-value assumption: Difference from the originalmean -0.9New observation record: Difference from the original mean 1No new-data replication verdict follows from these means alone
The calculations differ in their inputs or rule. Only the same-input, same-rule calculation is an exact recomputation of the original summary.
Read the diagram in words

The original and exact recomputation each use four/six/eight/zero and give 4.5. A zero substituted for the missing entry yields 3.6. New values five/seven/nine/one yield 5.5. The changed-rule difference is -0.9, and the new-record difference is one, with no declared replication verdict.

Calculation or evidence record
Calculation or evidence recordValues actually used by this calculationDenominator countNumerator totalCalculated mean
Original named summary4 · 6 · 8 · 04184.5
Same inputs and rule recomputed4 · 6 · 8 · 04184.5
Missing entry assumed to be zero4 · 6 · 8 · 0 · 05183.6
A new invented observation record5 · 7 · 9 · 14225.5
Difference from the original mean
Different missing-value assumptionNew observation record
-0.91

Original educational calculations only. The second result deliberately changes the treatment of a missing entry; the third uses new invented values. No empirical replication, uncertainty model or successful/failed consistency verdict is supplied.

02

Preserve chronology instead of rewriting the plan

A durable plan records the question, intended comparison and relevant analysis decisions before their outcomes are known. Prespecification helps a reader distinguish planned analysis from exploration prompted by a pattern in the results. It does not prevent useful discovery or ensure that every planned method was sensible.

The original timeline records four planned contrasts, then the observations, then a fifth contrast suggested by those observations. The fifth can be reported as exploratory. A documented amendment identifies when a rule changed, why and what information was already known. It does not move a result-driven choice backward in time.

Conditional analysis can be planned: a stated decision rule may select an appropriate method if a specified condition is met. That differs from choosing among results because one looks more convincing. Openly reporting justified deviations and exploratory analyses gives later readers a clearer basis for evaluating the claims.

The timing of an analysis decision changes its evidential role

The timing of an analysis decision changes its evidential roleA finite event sequence keeps four planned contrasts distinct from an exploratory comparison and a documented amendment.Sequence order, not elapsed time0. Question and four comparisons recordedWere the outcomes already known? NoPlanned comparison onePlanned comparison twoPlanned comparison threePlanned comparison four1. Assigned outcomes become availableWere the outcomes already known? Yes2. Four planned contrasts reportedWere the outcomes already known? YesPlanned comparison onePlanned comparison twoPlanned comparison threePlanned comparison four3. A fifth pattern suggests a comparisonWere the outcomes already known? YesResult-suggested comparison five4. Timing and reason for the new comparison documentedWere the outcomes already known? YesSpecified before the outcomes were knownSuggested after inspecting the outcomesA transparent change recordAn amendment does not rewrite the earlier chronology
Sequence positions indicate chronology, not elapsed durations. The amendment records a later change; it does not move the fifth comparison into the earlier plan.
Read the diagram in words

The plan records four contrasts before outcomes are known. Later, all four are reported. A fifth is suggested after inspection and retains its exploratory status; a subsequent amendment records that timing and reason. No event moves the fifth contrast into the earlier plan.

Recorded event
Recorded eventSequence order, not elapsed timeWere the outcomes already known?
Question and four comparisons recorded0No
Assigned outcomes become available1Yes
Four planned contrasts reported2Yes
A fifth pattern suggests a comparison3Yes
Timing and reason for the new comparison documented4Yes
Question and four comparisons recorded
Recorded event
Planned comparison one
Planned comparison two
Planned comparison three
Planned comparison four
Four planned contrasts reported
Recorded event
Planned comparison one
Planned comparison two
Planned comparison three
Planned comparison four
A fifth pattern suggests a comparison
Recorded event
Result-suggested comparison five
Recorded event
Recorded eventSpecified before the outcomes were known / Suggested after inspecting the outcomes
Planned comparison oneSpecified before the outcomes were known
Planned comparison twoSpecified before the outcomes were known
Planned comparison threeSpecified before the outcomes were known
Planned comparison fourSpecified before the outcomes were known
Result-suggested comparison fiveSuggested after inspecting the outcomes

A transparent change record

Invented ordered research-record events, not calendar durations, clinical registration rules, publication authority or proof that a plan removes bias. The sequence identifies what was known when a comparison was selected.

03

Evaluate a claim through its evidence and scope

A paper's title and reputation do not reveal how its measurements were obtained. Ask what question it actually addresses, which units were selected or assigned, how outcomes and exclusions were defined, and what assumptions connect its analysis to the conclusion. A review, an original study, an abstract and a protocol supply different kinds of information.

An abstract can establish that the authors report a finding and describe its stated scope, but it often cannot resolve all methodological details. A figure needs its definitions and underlying comparison; a numerical claim may require the original data or methods. Studies that repeat the same dataset do not supply independent new observations just because they appear in separate papers.

Collaboration also needs clear contributions and responsibilities. Records of who supplied data, developed an analysis, checked results and wrote an interpretation make the research easier to understand and correct. Appropriate credit, disclosed conflicts and respectful confidential review support that shared work; neither authorship nor independent review should be invented to fill a checklist.

Worked example

Separate three claims about checking

A same-input recomputation uses 4/6/8/0 and the retained eligibility rule. It again gives eighteen divided by four, 4.5. This is a check of the stated summary.

A different rule adds a zero for the missing record and gives 3.6. The -0.9 difference is explained by a changed denominator. A report can identify that discrepancy without claiming that the biology changed or that the first calculation was irreproducible.

A new four-value record 5/7/9/1 totals 22 and averages 5.5. The difference from 4.5 is one, but no sampling model, interval or consistency criterion for this new record is supplied. It is therefore new data, not an automatic successful or failed replication declaration.

Check your understanding

  1. What does an exact same-data recomputation establish in this example?

    Show reasoning

    It establishes that the named inputs and inclusion rule yield the reported summary again. It does not establish representative sampling, unbiased measurement or a valid causal conclusion.

  2. May a result-driven fifth contrast be reported after four contrasts were planned?

    Show reasoning

    Yes, with its exploratory status and timing clear. It should not be presented as if it had been prespecified before the observations were known.

  3. Why is one paper's abstract insufficient for every claim about its study design?

    Show reasoning

    The abstract reports a compressed account. Details about unit allocation, missingness, exclusions or analysis may require methods and underlying records. State the actual inspection extent rather than claiming a full review.

What to carry forward

Make the evidence relation explicit: same-data recomputation, new-data consistency and wider generalisation are separate claims.

References

Resources supporting this lesson’s explanations and examples.

  1. Reproducibility and Replicability in Science (2019; chapters 2 and 3; doi:10.17226/25303)National Academies of Sciences, Engineering, and MedicineCommittee reportnationalacademies.orgRead source
  2. Preregistration (Web guidance inspected 13 October 2026)Center for Open ScienceAgency conceptual guidancecos.ioRead source
  3. Singapore Statement on Research Integrity (2010; principles and selected responsibilities)Second World Conference on Research Integrity participants and statement drafting committeeInternational integrity statementwcrif.orgRead source